A SIMPLE AND RAPID METHOD FOR STAINING THE ENTERIC GANGLIA - APPLICATION FOR HIRSCHSPRUNGS-DISEASE

被引：9

作者：

HANANI, M

UDASSIN, R

ARIEL, I

FREUND, HR

机构：

[1] HADASSAH UNIV HOSP, DEPT PATHOL, IL-91240 JERUSALEM, ISRAEL

[2] HADASSAH UNIV HOSP, DEPT SURG, IL-91240 JERUSALEM, ISRAEL

来源：

JOURNAL OF PEDIATRIC SURGERY | 1993年 / 28卷 / 07期

关键词：

HIRSCHSPRUNGS DISEASE; MYENTERIC PLEXUS; SUBMUCOSAL PLEXUS; FLUORESCENT STAINING;

D O I：

10.1016/0022-3468(93)90702-M

中图分类号：

R72 [儿科学];

学科分类号：

100202 ;

摘要：

A simple and reliable method is described for staining the nerve plexuses of the human gastrointestinal tract in whole-mounts. The staining does not require fixation and consists of a single step of 6 to 10 minutes incubation in a solution of the fluorescent dye 4-(4-diethylaminostyryl)-methylpyridinium iodide. Aganglionic bowel specimens from 6 patients with Hirschsprung's disease were stained by this method and displayed the absence of ganglion cells and an abundance of nerve fibers in the myenteric and submucosal plexuses. This staining may be suitable for the diagnosis of Hirschsprung's disease. © 1993.

引用

页码：939 / 941

页数：3

共 11 条

[1] RECTAL MUCOSAL BIOPSY IN AGANGLIONOSIS AND ALLIED CONDITIONS [J].