SEQUENCING OF OLIGONUCLEOTIDE PHOSPHOROTHIOATES BASED ON SOLID-SUPPORTED DESULFURIZATION

被引：12

作者：

WYRZYKIEWICZ, TK

COLE, DL

机构：

[1] ISIS Pharmaceuticals, Carlsbad Research Center, Carlsbad, CA 92008

来源：

NUCLEIC ACIDS RESEARCH | 1994年 / 22卷 / 13期

关键词：

D O I：

10.1093/nar/22.13.2667

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We described a solid-supported desulfurization procedure allowing easy access to the sequence analysis of oligonucleotide phosphorothioates. The described method is based upon selective removal of the 2-cyanoethyl phosphate protecting groups, followed by iodine-promoted desulfurization of the resulting phosphorothioate diesters. Automatic oxidation of oligonucleotide phosphorothioates, anchored via an ester linkage to a standard solid support (LCAA/CPG), is combined with Maxam-Gilbert solid-support sequencing. The overall procedure allows rapid simultaneous sequence analysis of several oligonucleotide analogs.

引用

收藏

页码：2667 / 2669

页数：3

相关论文

共 19 条

[1] HINDERED DIALKYLAMINO NUCLEOSIDE PHOSPHITE REAGENTS IN THE SYNTHESIS OF 2 DNA 51-MERS [J].

ADAMS, SP ;

KAVKA, KS ;

WYKES, EJ ;

HOLDER, SB ;

GALLUPPI, GR .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1983, 105 (03) :661-663

[2] IMAGING PLATE ILLUMINATES MANY FIELDS [J].

AMEMIYA, Y ;

MIYAHARA, J .

NATURE, 1988, 336 (6194) :89-90

[3] THE SYNTHESIS OF MODIFIED OLIGONUCLEOTIDES BY THE PHOSPHORAMIDITE APPROACH AND THEIR APPLICATIONS [J].

BEAUCAGE, SL ;

IYER, RP .

TETRAHEDRON, 1993, 49 (28) :6123-6194

[4]

BIERGARDE K, 1991, NUCLEIC ACIDS RES, V19, P5843

[5] STEREOCHEMISTRY OF INTERNUCLEOTIDE BOND FORMATION BY POLYNUCLEOTIDE PHOSPHORYLASE FROM MICROCOCCUS-LUTEUS [J].

BURGERS, PMJ ;

ECKSTEIN, F .

BIOCHEMISTRY, 1979, 18 (03) :450-454

[6]

CONOLLY BA, 1984, BIOCHEMISTRY-US, V23, P3443

[7]

COOK PD, 1993, MED CHEM STRATEGIES, P150

[8] STEREOCHEMICAL EVIDENCE FOR A PHOSPHORYLPYRIDINIUM INTERMEDIATE IN THE IODINE-MEDIATED DESULFURIZATION OF A PHOSPHOROTHIOATE DIESTER [J].

CUMMINS, JH ;

POTTER, BVL .

JOURNAL OF THE CHEMICAL SOCIETY-CHEMICAL COMMUNICATIONS, 1985, (12) :800-802

[9] SOLID SUPPORTED HYDROLYSIS OF APURINIC SITES IN SYNTHETIC OLIGONUCLEOTIDES FOR RAPID AND EFFICIENT PURIFICATION ON REVERSE-PHASE CARTRIDGES [J].

HORN, T ;

URDEA, MS .

NUCLEIC ACIDS RESEARCH, 1988, 16 (24) :11559-11571

[10] 3H-1,2-BENZODITHIOLE-3-ONE 1,1-DIOXIDE AS AN IMPROVED SULFURIZING REAGENT IN THE SOLID-PHASE SYNTHESIS OF OLIGODEOXYRIBONUCLEOSIDE PHOSPHOROTHIOATES [J].

IYER, RP ;

EGAN, W ;

REGAN, JB ;

BEAUCAGE, SL .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1990, 112 (03) :1253-1254