SIMPLE METHOD OF PURIFICATION OF ESCHERICHIA-COLI HEAT-LABILE ENTEROTOXIN AND CHOLERA-TOXIN USING IMMOBILIZED GALACTOSE

被引：84

作者：

UESAKA, Y

OTSUKA, Y

LIN, Z

YAMASAKI, S

YAMAOKA, J

KURAZONO, H

TAKEDA, Y

机构：

[1] KYOTO UNIV,FAC MED,DEPT MICROBIOL,SAKYO KU,KYOTO 60601,JAPAN

[2] NISSUI PHARMACEUT CO,INST DIAGNOST REAGENTS,YUKI,IBARAKI 307,JAPAN

来源：

MICROBIAL PATHOGENESIS | 1994年 / 16卷 / 01期

关键词：

HEAT-LABILE ENTEROTOXIN; CHOLERA TOXIN; PURIFICATION;

D O I：

10.1006/mpat.1994.1007

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

A simple method for purification of heat-labile enterotoxin produced by enterotoxigenic Escherichia coli and for purification of cholera toxin using immobilized D-galactose column is described. A single run of column chromatography yielded homogeneous toxin as demonstrated by sodium dodecyl sulfate polyacrylamide slab gel electrophoresis. © 1994 Academic Press.

引用

页码：71 / 76

页数：6

共 16 条

[1] ISOLATION AND CHARACTERIZATION OF HOMOGENEOUS HEAT-LABILE ENTEROTOXINS WITH HIGH SPECIFIC ACTIVITY FROM ESCHERICHIA-COLI CULTURES [J].