A ROLE IN DNA-BINDING FOR THE LINKER SEQUENCES OF THE 1ST 3 ZINC FINGERS OF TFIIIA

被引：94

作者：

CHOO, Y

KLUG, A

机构：

[1] Medical Research Council, Laboratory of Molecular Biology

来源：

NUCLEIC ACIDS RESEARCH | 1993年 / 21卷 / 15期

基金：

英国医学研究理事会;

关键词：

D O I：

10.1093/nar/21.15.3341

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Zinc fingers of the TFIIIA type are connected by short linker sequences between the structural units. Structural investigations by 2D NMR in solution and by X-ray crystallographic analyses of complexes with DNA point to a passive role for the linkers. We have therefore investigated the influence of the linker sequence on DNA binding using as a model the first three fingers of the protein TFIIIA. Insertion of certain heterologous linkers abolishes binding, and replacement of individual amino acids can reduce binding by tactors of up to twenty-tour.

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收藏

页码：3341 / 3346

页数：6

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