RECOMBINANT SOLUBLE UROKINASE RECEPTOR AS A SCAVENGER FOR UROKINASE-TYPE PLASMINOGEN-ACTIVATOR (UPA) - INHIBITION OF PROLIFERATION AND INVASION OF HUMAN OVARIAN-CANCER CELLS

被引：110

作者：

WILHELM, O ^{[1
]}

WEIDLE, U ^{[1
]}

HOHL, S ^{[1
]}

RETTENBERGER, P ^{[1
]}

SCHMITT, M ^{[1
]}

GRAEFF, H ^{[1
]}

机构：

[1] BOEHRINGER MANNHEIM GMBH,D-82377 PENZBERG,GERMANY

来源：

FEBS LETTERS | 1994年 / 337卷 / 02期

关键词：

UROKINASE RECEPTOR; INVASION; PROLIFERATION; OVARIAN CANCER;

D O I：

10.1016/0014-5793(94)80259-9

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

A recombinant soluble human urokinase receptor comprising amino acids 1-277 was cloned and transfected into CHO cells. The mutant protein (rec-uPAR(277)), purified from the CHO cell supernatant by affinity chromatography on immobilized urokinase (uPA), in a four-fold excess, completely abolished the binding of FITC-labeled pro-uPA to the human ovarian cancer cell line, OV-MZ-6. This invasive and tumorigenic cancer cell line expresses uPA, its inhibitor PAI-1, and the high-affinity receptor for uPA, uPAR. Rec-uPAR(277) significantly reduced the proliferation of OV-MZ-6 cells in a concentration-dependent manner without altering the viability of the cells. Invasion of OV-MZ-6 cells tested in an in vitro Matrigel invasion assay was inhibited by rec-uPAR(277) up to 75%. In conclusion, these results demonstrate that rec-uPAR(277) can function as a scavenger for uPA in vitro by inhibiting proliferation and invasion of human cancer cells.

引用

页码：131 / 134

页数：4

共 20 条

[1]

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[2]

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