FUMARATE PERMEATION IN RAT-LIVER MITOCHONDRIA - FUMARATE MALATE AND FUMARATE PHOSPHATE TRANSLOCATORS

被引：19

作者：

ATLANTE, A ^{[1
]}

PASSARELLA, S ^{[1
]}

GIANNATTASIO, S ^{[1
]}

QUAGLIARIELLO, E ^{[1
]}

机构：

[1] UNIV BARI, CNR, CTR STUDIO MITOCONDRI & METAB ENERGET, I-70126 BARI, ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1985年 / 132卷 / 01期

关键词：

D O I：

10.1016/0006-291X(85)90981-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

070307 [化学生物学]; 071010 [生物化学与分子生物学];

摘要：

Fumarate permeation in isolated rat liver mitochondria was demonstrated by measuring malate and phosphate efflux caused by fumarate added externally to the mitochondrial suspension. The existence of two specific fumarate translocators, fumarate/malate and fumarate/phosphate, is shown here. These carriers are distinguished in the light of different kinetic parameters (Km values are 50 .mu.M and 150 .mu.M, and Vmax values are 17 and 40 nmoles/min .times. mg mitochondrial protein, respectively) and of differing sensitivity to non-penetrant compounds. Fumarate was found to cause oxaloacetate efflux from mitochondria by means of an indirect process which involves the cooperation of both fumarate/malate and malate/oxaloacetate translocators. Results are discussed in the light of the physiological role played by fumarate translocation in both ureogenesis and aminoacid metabolism.

引用

页码：8 / 18

页数：11

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