MIGRATORY ACTIVITY OF HUMAN SMOOTH-MUSCLE CELLS CULTIVATED FROM CORONARY AND PERIPHERAL PRIMARY AND RESTENOTIC LESIONS REMOVED BY PERCUTANEOUS ATHERECTOMY

Background. The successful cultivation of human smooth muscle cells (SMC) from coronary and peripheral atherosclerotic lesions removed by percutaneous directional atherectomy is described. Methods and Results. Sixty-seven patients in whom plaque material was obtained compose the study population. A total of 73 lesions from both coronary (n = 38) and peripheral (n = 35) arteries of primary (n = 50) and restenotic origin (n = 23) were studied. Successful cultivation was significantly (p < 0.001) dependent on the quantity of plaque material submitted. Fifty-five percent of patients in whom atherectomy specimens were removed from coronary lesions yielded an adequate SMC population in comparison to 89% of those from peripheral arteries (p < 0.01). Cultivation was not dependent on the age and sex of patients, lesion origin, risk factors, medications, or incidence of unstable angina. In an attempt to quantify SMC activity, migratory velocity was measured with a computer-assisted motion analysis system. SMC migratory velocity was found to be significantly (p < 0.001) greater in restenotic than in primary plaque material. This finding was confirmed for both coronary and peripheral lesions. Conclusions. Our data suggest that elevated SMC migratory activity may be an important mechanism in the development of restenotic lesions.