LIPOPOLYSACCHARIDE CORE REGION OF HAFNIA-ALVEI - STRUCTURE ELUCIDATION USING CHEMICAL METHODS, GAS-CHROMATOGRAPHY MASS-SPECTROMETRY, AND NMR-SPECTROSCOPY

Sugar and methylation analysis with the use of gas chromatography-mass spectrometry and H-1 NMR spectroscopy proved that the core oligosaccharides isolated from lipopolysaccharides of eight Hafnia alvei strains have the identical hexasaccharide skeleton. However, H-1,P-31 heterocorrelated spectra showed that the phosphorylation pattern is not the same. The branched heptose for the ATCC 13337, 1187, 2, 1191, 1196, 1220, and 481L strains is phosphorylated as in the following formula,where P = -O-P(O)(O-)(2) and P-PEtN = [-O-P(O)(O-)](2)-O(CH2)(2)NH3+ [GRAPHICS] A different phosphorylation pattern was found for the 1211 strain, where the branched heptose residue is 6-substituted by a monophosphorylethanolamine group, ...-->3(-->7)(PEtN-->6)-alpha-LD-Hepp-(1-->3)..., where PEtN = -O-P(O)(O-)-O(CH2)(2)NH3+.