A TUNGSTEN-IN-GLASS IONTOPHORESIS ASSEMBLY FOR STUDYING INPUT-OUTPUT RELATIONSHIPS IN CENTRAL NEURONS

A method is described for the production of an electrode capable of monitoring and modulating the input-output relationship of thalamic neurons. Tungsten-in-glass electrodes were manufactured with the ability to simultaneously record lateral geniculate nucleus action potentials and associated, retinally evoked S-potentials. The recording electrodes were mounted onto multibarreled micropipettes with iontophoretic capability. The completed electrode assembly permitted micropharmacological modulation of the fraction of lateral geniculate nucleus output spikes to retinal input spikes (the transfer ratio). Iontophoretically applied gamma-aminobutyric acid (GABA) decreased the transfer ratio, an effect countered by the GABA(A) antagonist, bicuculline. Elevated transfer ratios produced by stimulation of an afferent pathway originating in the parabrachial region of the brainstem were decreased by concurrently applied GABA. The fabrication of this electrode assembly employs simple modifications of existing techniques and separate construction of recording and iontophoretic elements to provide high-quality single-unit recordings coupled with micropharmacological function.