ASSAY OF URINARY FREE AND CONJUGATED 3-METHOXY-4-HYDROXYPHENYLETHYLENEGLYCOL BY HIGH-PERFORMANCE LIQUID-CHROMATOGRAPHY WITH AMPEROMETRIC DETECTION

被引：6

作者：

BOURDEAUX, R

CAPOLAGHI, B

LACROIX, C

YOUNOS, C

LEHR, PR

机构：

[1] CHR THIONVILLE,HOP BEL AIR,BIOL LAB,F-57100 THIONVILLE,FRANCE

[2] CTR HOSP LE HAVRE,PHARMACOCINET LAB,F-76600 LE HAVRE,FRANCE

[3] UNIV METZ,CTR ENVIRONM SCI,F-57000 METZ,FRANCE

[4] UNIV NANCY 1,FAC SCI,BIOL & PHYSIOL COMPORTEMENT LAB,CNRS,URA 1293,F-54000 NANCY,FRANCE

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1995年 / 665卷 / 01期

关键词：

D O I：

10.1016/0378-4347(94)00516-8

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

The aim of this study was to develop an analytical method for free and conjugated 3-methoxy-4-hydroxyphenylethyleneglycol (MHPG) in urine. After hydrolysis of the conjugated forms, the urinary MHPG was purified by solid-phase extraction on anion exchanger and eluted with a water-methanol (1:1, v/v) mixture. After addition of ethyl acetate to the eluate and back-extraction into acetic acid, the aqueous phase was separated on a C-18 column by HPLC and detected amperometrically. The results obtained from forty healthy human subjects were compared with the literature values. The precision and accuracy of the assay were studied using 4-methoxy-3-hydroxyphenylethyleneglycol (iso-MHPG) as internal standard.

引用

收藏

页码：45 / 51

页数：7

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