THE EFFECTS OF CHOLESTEROL DEPLETION ON THE SODIUM-PUMP IN HUMAN RED-CELLS

被引：13

作者：

LUCIO, FJ ^{[1
]}

HENDRY, BM ^{[1
]}

ELLORY, JC ^{[1
]}

机构：

[1] JOHN RADCLIFFE HOSP,NUFFIELD DEPT CLIN MED,OXFORD OX3 9DU,ENGLAND

来源：

EXPERIMENTAL PHYSIOLOGY | 1991年 / 76卷 / 03期

关键词：

D O I：

10.1113/expphysiol.1991.sp003510

中图分类号：

Q4 [生理学];

学科分类号：

071003 ;

摘要：

Sodium pump function has been studied in human erythrocytes depleted of membrane cholesterol by incubation with phosphatidylcholine liposomes. The cells were sodium loaded by incubation in alkaline sodium phosphate and sodium pump activity was assessed by measurements of ouabain-sensitive Rb-86 uptake at 37-degrees-C. Cholesterol depletion had a biphasic effect; depletion by 5-25% increased sodium pump activity by a mean of 16.1% (S.D. 3.2%), whereas depletion by 35-50% decreased sodium pump activity by a mean of 14.8% (S.D. 3.8%). Cholesterol depletion had no reproducible effect on the ouabain-insensitive uptake of Rb. These results support the hypothesis that there may be an optimum membrane cholesterol content for sodium pump function.

引用

页码：437 / 443

页数：7

共 19 条

[1]

CLARET M, 1978, J PHYSIOL-LONDON, V274, P247, DOI 10.1113/jphysiol.1978.sp012145

[2]

DEPONT JJHHM, 1983, CURR TOP MEMBR TRANS, V19, P163

[3]

DEUTICKE B, 1987, ANNU REV PHYSIOL, V49, P221

[4] EFFECTS OF DIALYSIS AND TRANSPLANTATION ON RED-CELL NA PUMP FUNCTION IN RENAL-FAILURE [J].

FERVENZA, FC ;

HENDRY, BM ;

ELLORY, JC .

NEPHRON, 1989, 53 (02) :121-128

[5]

GARAY RP, 1987, HYPERTENSION S1, V10, P11

[6] THE EFFECTS OF MEMBRANE LIPID ORDER AND CHOLESTEROL ON THE INTERNAL AND EXTERNAL CATIONIC SITES OF THE NA+-K+ PUMP IN ERYTHROCYTES [J].

GIRAUD, F ;

CLARET, M ;

BRUCKDORFER, KR ;

CHAILLEY, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1981, 647 (02) :249-258

[7] INTERACTIONS OF CHOLESTEROL WITH NA PUMP IN RED BLOOD-CELLS [J].

GIRAUD, F ;

CLARET, M ;

GARAY, R .

NATURE, 1976, 264 (5587) :646-648

[8]

GRUNDY SM, 1988, NEW ENGL J MED, V319, P24

[9] DUAL EFFECT OF MEMBRANE CHOLESTEROL ON SIMPLE AND MEDIATED TRANSPORT PROCESSES IN HUMAN-ERYTHROCYTES [J].

GRUNZE, M ;

FORST, B ;

DEUTICKE, B .

BIOCHIMICA ET BIOPHYSICA ACTA, 1980, 600 (03) :860-869

[10]

KROES J, 1971, BIOCHIM BIOPHYS ACTA, V249, P247

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