MOLECULAR-CLONING AND DNA-SEQUENCE ANALYSIS OF CDNA-ENCODING CHICKEN HOMOLOG OF THE BCL-2-ONCOPROTEIN

被引：37

作者：

CAZALSHATEM, DL ^{[1
]}

LOUIE, DC ^{[1
]}

TANAKA, S ^{[1
]}

REED, JC ^{[1
]}

机构：

[1] UNIV PENN,DEPT PATHOL & LAB MED,PHILADELPHIA,PA 19104

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1992年 / 1132卷 / 01期

关键词：

BCL-2; ONCOPROTEIN; MITOCHONDRION; NUCLEOTIDE SEQUENCE; APOPTOSIS; PROGRAMMED CELL DEATH; (CHICKEN);

D O I：

10.1016/0167-4781(92)90064-7

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a 2228 bp cDNA clone encoding a chicken homologue of the human Bcl-2 oncoprotein by low-stringency hybridization screening of a lambda-gt10 cDNA library derived from a chicken B-cell lymphoma. DNA sequence analysis of this cDNA revealed an open reading frame predicting a polypeptide of 232 amino acids and an M(r) of 25 839. The predicted protein is highly homologous to the human (73%) and mouse (70%) Bcl-2 proteins, and contains a hydrophobic stretch of amino acids within its carboxyl-end (213-229) consistent with an integral membrane protein. Areas of very high sequence homology shared by all three Bcl-2 proteins at the NH2-terminus (amino acids 1-33) and within the last 150 amino acids of these proteins suggest the presence of at least two evolutionarily conserved domains within the family of Bcl-2 proteins that may be important either for their targeting to mitochondria or their ability to block programmed cell death.

引用

页码：109 / 113

页数：5

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