DIFFERENTIATION OF CA2+-COORDINATED AND MG2+-COORDINATED BRANCHED TRISACCHARIDE ISOMERS - AN ELECTROSPRAY-IONIZATION AND TANDEM MASS-SPECTROMETRY STUDY

被引:112
作者
FURA, A [1 ]
LEARY, JA [1 ]
机构
[1] UNIV CALIF BERKELEY,DEPT CHEM,BERKELEY,CA 94720
关键词
D O I
10.1021/ac00068a017
中图分类号
O65 [分析化学];
学科分类号
070302 ; 081704 ;
摘要
Three isomeric branched trisaccharides coordinated to calcium and magnesium were analyzed by electrospray ionization followed by tandem mass spectrometry. Studies were undertaken to optimize the singly charged metal coordinated precursor prior to MS/MS and MS/MS/MS experiments. The positioning of the glycosidic linkages between reducing and nonreducing rings could be determined in all three isomers, and the linkage of the fucose ring was obvious in two of the three isomers when calcium was used as the coordinating metal. The ionic radius and charge of the metal appear to be very important in obtaining structural information in these isomers.
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页码:2805 / 2811
页数:7
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