BILE SECRETION OF TRACE-ELEMENTS IN RATS WITH A CONGENITAL DEFECT IN HEPATOBILIARY TRANSPORT OF GLUTATHIONE

被引:24
作者
DIJKSTRA, M
KUIPERS, F
HAVINGA, R
SMIT, EP
VONK, RJ
机构
[1] Department of Pediatrics, University of Groningen, Groningen, KZ, 9712
关键词
D O I
10.1203/00006450-199010000-00008
中图分类号
R72 [儿科学];
学科分类号
100202 ;
摘要
Bile secretion of trace elements, analyzed by proton-induced x-ray emission, was studied in rats with a congenital defect in hepatobiliary transport of organic anions [Groningen Yellow (GY) rats], in which the process of bile secretion resembles that of the neonatal period. Bile flow (-41%) and biliary glutathione secretion (-99%) were drastically impaired in GY rats compared with controls. Plasma concentrations of all detectable trace elements (Fe, Cu, Zn, Mo, Br, and Se), as well as that of simultaneously determined Ca, were similar in GY and age-matched control Wistar rats. Bile concentrations of Fe, Mo, Br, and Ca were also similar in both groups, resulting in a −40% reduction of their secretion rates in GY rats. The concentrations of Zn (-62%) and Mn (-64%) were significantly lower in GY rats in contrast to that of Cu, which was 50% higher. Se could not be detected in bile of either group. Recovery in bile (% dose/3 h) after i.v. injection of MnCl2, CuSO4, or SeO2 (1 mg metal/kg) was lower in GY rats than in controls: Mn, 26 and 35%; Cu, 2.6 and 5%; and Se, 1.5 and 5%, respectively. Injection of ZnSCO4 did not lead to increased Zn secretion in GY rats, and only 1.1% of the dose was recovered in controls. Thus, the hepatic handling of different endogenous and exogenously administered trace metals is affected to a variable extent in the GY rat. For a number of metals (e.g. Fe, Mo), this may be related to the reduced bile flow; for others (e.g. Zn, Mn, Cu), other regulatory factors appear to be responsible. © 1990 International Pediatric Research Foundation, Inc.
引用
收藏
页码:339 / 343
页数:5
相关论文
共 33 条
[1]   BILIARY TRANSPORT OF GLUTATHIONE DISULFIDE STUDIED WITH ISOLATED RAT-LIVER CANALICULAR-MEMBRANE VESICLES [J].
AKERBOOM, T ;
INOUE, M ;
SIES, H ;
KINNE, R ;
ARIAS, IM .
EUROPEAN JOURNAL OF BIOCHEMISTRY, 1984, 141 (01) :211-215
[2]  
ALEXANDER J, 1981, ACTA PHARMACOL TOX, V49, P190
[3]   BILIARY-EXCRETION OF COPPER AND ZINC IN THE RAT AS INFLUENCED BY DIETHYLMALEATE, SELENITE AND DIETHYLDITHIOCARBAMATE [J].
ALEXANDER, J ;
AASETH, J .
BIOCHEMICAL PHARMACOLOGY, 1980, 29 (15) :2129-2133
[4]   HEPATOBILIARY TRANSPORT AND ORGAN DISTRIBUTION OF SILVER IN THE RAT AS INFLUENCED BY SELENITE [J].
ALEXANDER, J ;
AASETH, J .
TOXICOLOGY, 1981, 21 (03) :179-186
[5]   ROLE OF THE LIVER IN NORMAL IRON-METABOLISM [J].
BACON, BR ;
TAVILL, AS .
SEMINARS IN LIVER DISEASE, 1984, 4 (03) :181-192
[6]  
BALISTRERI WF, 1983, J PEDIATR GASTR NUTR, V2, pS207, DOI 10.1097/00005176-198300201-00030
[7]  
BALLARORI N, 1989, AM J PHYSIOL, V256, pG1
[8]   DEVELOPMENTAL-CHANGES IN THE BILIARY-EXCRETION OF METHYLMERCURY AND GLUTATHIONE [J].
BALLATORI, N ;
CLARKSON, TW .
SCIENCE, 1982, 216 (4541) :61-63
[9]   BILIARY-SECRETION OF GLUTATHIONE AND OF GLUTATHIONE METAL-COMPLEXES [J].
BALLATORI, N ;
CLARKSON, TW .
FUNDAMENTAL AND APPLIED TOXICOLOGY, 1985, 5 (05) :816-831
[10]   PIXE TRACE-ELEMENT DETERMINATION AND ITS ACCURACY IN THE ANALYSIS OF BILE [J].
BOERMA, DO ;
SMIT, EP ;
ROOSNEK, N .
NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS, 1989, 36 (01) :60-73