SILVER-STAINED STRUCTURES IN PROSTATIC-CARCINOMA - EVALUATION OF DIAGNOSTIC AND PROGNOSTIC RELEVANCE BY AUTOMATED IMAGE-ANALYSIS

被引：29

作者：

CONTRACTOR, H ^{[1
]}

RUSCHOFF, J ^{[1
]}

HANISCH, T ^{[1
]}

ULSHOFER, B ^{[1
]}

NEUMANN, K ^{[1
]}

SCHULTZESEEMANN, W ^{[1
]}

THOMAS, C ^{[1
]}

机构：

[1] UNIV MARBURG,ZENTRUM PATHOL,BALDINGERSTR,W-3550 MARBURG,GERMANY

来源：

UROLOGIA INTERNATIONALIS | 1991年 / 46卷 / 01期

关键词：

NUCLEOLAR ORGANIZER REGIONS; SILVER STAIN METHOD; PROSTATIC CARCINOMA; PROGNOSIS OF; AUTOMATED IMAGE ANALYSIS;

D O I：

10.1159/000281764

中图分类号：

R5 [内科学]; R69 [泌尿科学（泌尿生殖系疾病）];

学科分类号：

1002 ; 100201 ;

摘要：

The comparison of the diagnostic and prognostic significance of histology, immunohistochemical parameters (PSA, PSP), and silver-stained nucleolar organizer regions (AgNORs) was estimated in paraffin sections taken of 63 prostatic carcinomas prior to therapy. AgNORs were visualized with a one-step silver staining technique with the appropiate staining time determined by preliminary staining-time series. The mean AgNOR number per cell (n) and the mean AgNOR area per silver-stained dot (A) were determined by means of an automatic image analysis system. Thereby prostatic carcinomas exhibited multiple small AgNORs within their nuclei (n = 4.7, A = 0.09-mu-m2), whereas benign prostatic epithelium showed few but large silver-stained particles (n = 1.8, A = 0.27-mu-m2; p < 0.001). This relationship was then calculated as a quotient of ArNOR number and area (NQ = n/A) which provided additional information for the diagnosis of malignancy as well as survival. Univariate survival analysis disclosed a set of four variables predicting death from prostatic cancer: cribriform growth pattern, AgNOR quotient, histological grade, and PSA immunoreactivity. Of these parameters, immunoreactivity of PSA failed to prove its prognostic significance in multivariate survival analysis (Cox model). No relation to prognosis was found for the number as well as the area of AgNORs alone. Therefore, image analysis proved to be a prerequisit for the feasibility of this promising technique by providing objective and reproducible results.

引用

页码：9 / 14

页数：6

共 43 条

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