MECHANISM-BASED STRATEGIES FOR PROTEIN THERMOSTABILIZATION

被引：231

作者：

MOZHAEV, VV

机构：

[1] Department of Chemistry, Moscow State University

来源：

TRENDS IN BIOTECHNOLOGY | 1993年 / 11卷 / 03期

关键词：

D O I：

10.1016/0167-7799(93)90057-G

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

Strategies for stabilizing enzymes can be derived from a two-step model of irreversible inactivation that involves preliminary reversible unfolding, followed by an irreversible step. Reversible unfolding is best prevented by covalent immobilization, whereas methods such as covalent modification of amino acid residues or 'medium engineering' (by the addition of low-molecular-weight compounds) are effective against irreversible 'incorrect' refolding. Genetic modification of the protein sequence is the most effective approach for preventing chemical deterioration.

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页码：88 / 95

页数：8

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