AMIDE HYDROGEN-EXCHANGE OF THE CENTRAL B-CHAIN HELIX WITHIN THE T-STATES AND R-STATES OF INSULIN HEXAMERS

被引:5
作者
HARDAWAY, LA
BREMS, DN
BEALS, JM
MACKENZIE, NE
机构
[1] UNIV ARIZONA,DEPT PHARMACEUT SCI,TUCSON,AZ 85721
[2] ELI LILLY & CO,LILLY CORP CTR,LILLY RES LABS,INDIANAPOLIS,IN 46285
来源
BIOCHIMICA ET BIOPHYSICA ACTA-PROTEIN STRUCTURE AND MOLECULAR ENZYMOLOGY | 1994年 / 1208卷 / 01期
关键词
INSULIN; EXCIPIENT; NMR; H-1-; AMIDE EXCHANGE; ALLOSTERIC CONFORMATION;
D O I
10.1016/0167-4838(94)90165-1
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Comparative analysis of the H-1-NMR spectra of human insulin shows that in the presence of the allosteric ligand, phenol, the tertiary structure of the protein is altered as evidenced by the decreased rate of amide hydrogen-deuterium exchange. In particular, exchange of amide protons in residues of the B-chain helix (B-9-B-20) are significantly affected suggesting either a stabilization of this helix or a reduction in the solvent accessibility of the helix in the R-state. This paper exemplifies the exchange rates of two amides (Val(B18) and Tyr(B16)) from this helix which decrease by approximately 400-fold as a result of this ligand induced conformational transition.
引用
收藏
页码:101 / 103
页数:3
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