GLYCOPROTEINS SPECIFIC FOR THE RETINAL ROD OUTER SEGMENT PLASMA-MEMBRANE

被引：34

作者：

MOLDAY, LL ^{[1
]}

MOLDAY, RS ^{[1
]}

机构：

[1] UNIV BRITISH COLUMBIA, DEPT BIOCHEM, VANCOUVER V6T 1W5, BC, CANADA

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1987年 / 897卷 / 02期

关键词：

D O I：

10.1016/0005-2736(87)90430-5

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

Two ricin-specific glycoproteins have been identified on neuraminidase-treated rod outer segment plasma membranes of bovine retinal photoreceptor cells. Ricin-gold-dextran particles were observed by electron microscopy to density label the surface of neuraminidase-treated rod outer segments. Western blotting of proteins separated by SDS-gel electrophoresis indicated that two ricin-binding glycoproteins of Mr 230000 and 110000 are specific for the plasma membrane and are not found in disk membranes. These glycoproteins can serve as specific probes for the purification of the rod outer segment plasma membranes.

引用

页码：335 / 340

页数：6

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