ESTABLISHMENT AND CHARACTERIZATION OF HUMAN CARCINOEMBRYONIC ANTIGEN TRANSGENIC MICE

被引:20
作者
HASEGAWA, T
ISOBE, K
TSUCHIYA, Y
OIKAWA, S
NAKAZATO, H
IKEZAWA, H
NAKASHIMA, I
SHIMOKATA, K
机构
[1] SUNTORY INST BIOMED RES,OSAKA 618,JAPAN
[2] NAGOYA CITY UNIV,FAC PHARMACEUT SCI,MIZUHO KU,NAGOYA,AICHI 467,JAPAN
[3] NAGOYA UNIV,SCH MED,DEPT IMMUNOL,NAGOYA,AICHI 466,JAPAN
[4] NAGOYA UNIV,SCH MED,DEPT INTERNAL MED 1,65 TSURUMAI CHO,SHOWA KU,NAGOYA,AICHI 466,JAPAN
关键词
D O I
10.1038/bjc.1991.386
中图分类号
R73 [肿瘤学];
学科分类号
100214 ;
摘要
We have produced human CEA transgenic mice which were found to express CEA mRNA in all tissues. By immunoblot analysis using anti-CEA polyclonal antibody, we also detected CEA protein in all tissues. However, the molecular size of CEA in the brain was different from that in other tissues, although the mRNA size was same and no deletion nor rearrangement was detected at the DNA level. Immunohistochemical analysis of the lung and the colon showed that the expression sites were the bronchial epithelial cells of the lung and the columnar epithelial cells of the colon. Interestingly, the expression of CEA protein in the transgenic mice was polarised to the luminal side of epithelial cells similar to the normal CEA expression in human tissues. We also detected cell surface expression of human CEA on thymocytes and spleen cells and CEA expression was greatly reduced by the phosphatidylinositol-specific phospholipase C (PI-PLC) treatment.
引用
收藏
页码:710 / 714
页数:5
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