A RAPID METHOD FOR DETERMINATION OF THE ASSOCIATION CONSTANT OF MONOCLONAL-ANTIBODIES

被引：6

作者：

KATOH, S

SOHMA, Y

TERASHIMA, M

SADA, E

TSUGAWA, M

机构：

[1] Department of Synthetic Chemistry and Biological Chemistry, Kyoto University, Kyoto

来源：

JOURNAL OF FERMENTATION AND BIOENGINEERING | 1993年 / 76卷 / 06期

关键词：

D O I：

10.1016/0922-338X(93)90239-5

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

A rapid ELISA method for measuring association constants of monoclonal antibodies against protein antigens is proposed. Two microtiter plates were used; one plate was used for equilibrating monoclonal antibody solutions of various concentrations with antigens in wells of the plate and the other for measuring the equilibrated concentrations of the antibody by ELISA. It was possible to determine the association constants within a few hours by this method and apply the method to association constants larger than those measured by the equilibrium dialysis method.

引用

页码：451 / 454

页数：4

共 6 条

[1] CONFORMATIONAL STUDIES ON MODIFIED PROTEINS AND PEPTIDES - ARTIFICIAL MYOGLOBINS PREPARED WITH MODIFIED AND METALLOPORPHYRINS [J].