PROPERTIES AND REGULATION OF BASOLATERAL K+ CHANNELS IN RAT DUODENAL CRYPTS

Patch clamp recording techniques were used to study the properties of K+ channels in the basolateral membrane of rat duodenal crypts, and their regulation by Cl--secretory agonists. 2. High conductance (84-99 pS) K+ channels were activated by 0.1 mM dibutyryl cAMP in 50 % of cell-attached patches, while 0.1 mM carbachol had no effect on channel activity. High conductance K+ channels were voltage independent, Ca2+ insensitive, blocked by 5 mM Ba2+ or 20 mM tetraethylammonium chloride (TEA), and stimulated by 57 % when intracellular pH was increased from 7.4 to 7.8. 3. In contrast, low conductance (19-28 pS) K+ channels mere activated by 0.1 mM dibutyryl cAMP in 67 % of cell-attached patches, while 0.1 mM carbachol activated channels in 100 % of cell-attached patches. Low conductance Kf channels were voltage independent, Ca2+ sensitive and pH insensitive. Unlike the high canductance K+ channels, 5 mM Ba2+ had no effect on the activity of low conductance Kf channels, although 20 mM TEA decreased channel activity by 53 %. 4. The results show that the basolateral membrane of rat duodenal crypts possesses two discrete populations of K+ channels that may have important roles in sustaining the small intestinal Cl--secretory responses triggered by a variety of cAMP- and Ca2+ mediated agonists.