MONOCLONAL-ANTIBODIES TO OXYTOCIN - PRODUCTION AND CHARACTERIZATION

被引：16

作者：

BURGEON, E

CHAPLEUR, M

SCHOENEN, J

REMICHIUS, D

LEGROS, JJ

GEENEN, V

ROBERT, F

机构：

[1] UNIV LIEGE SART TILMAN,CHU LIEGE,NEUROENDOCRINE UNIT,RADIOIMMUNOL LAB,B-23,B-4000 LIEGE,BELGIUM

[2] FAC MED VANDOEUVRE NANCY,INSERM,U308,F-54500 VANDOEUVRE NANCY,FRANCE

[3] UNIV LIEGE SART TILMAN,DEPT NEUROPATHOL,B-4000 LIEGE,BELGIUM

[4] UNIV LIBRE BRUXELLES,PHYSIOL LAB,B-1640 RHODE ST GENESE,BELGIUM

来源：

JOURNAL OF NEUROIMMUNOLOGY | 1991年 / 31卷 / 03期

关键词：

HYBRIDOMA; NEUROHYPOPHYSEAL PEPTIDE; CONJUGATE; ENZYME-LINKED IMMUNOSORBENT ASSAY; RADIOIMMUNOASSAY; IMMUNOHISTOCHEMISTRY;

D O I：

10.1016/0165-5728(91)90045-9

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

To induce a good immune response to oxytocin (OT) we developed a two-step technique to conjugate OT to thyroglobulin (TG) using glutaraldehyde. We obtained 30 hybridomas recognizing OT-ovalbumin conjugates and 16 stable lines. Three monoclonal antibodies were selected for further characterization. One of them (O13) was found very specific for OT using three different techniques (enzyme-linked immunosorbent assay, radioimmunoassay and immunohistochemistry); it is directed to the C-terminal tripeptide. The other two probably recognize tyrosine containing epitope(s) also shared by vasopressin and other related nonapeptides.

引用

页码：235 / 244

页数：10

共 23 条

[1] STRUCTURAL REQUIREMENTS FOR RECOGNITION OF VASOPRESSIN BY ANTIBODY - THERMODYNAMIC AND KINETIC CHARACTERISTICS OF THE INTERACTION [J].