A QUANTITATIVE TEST FOR COPPER USING BICINCHONINIC ACID

被引：172

作者：

BRENNER, AJ

HARRIS, ED

机构：

[1] TEXAS A&M UNIV,DEPT BIOCHEM & BIOPHYS,COLLEGE STN,TX 77843

[2] TEXAS A&M UNIV,FAC NUTR,COLLEGE STN,TX 77843

来源：

ANALYTICAL BIOCHEMISTRY | 1995年 / 226卷 / 01期

关键词：

D O I：

10.1006/abio.1995.1194

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

We describe a direct colorimetric assay for copper in serum and biological samples using 2,2'-bicinchoninic acid (BCA), a common reagent in most laboratories. BCA offers the advantage of being highly sensitive and specific for Cu(I) which rapidly forms an intense purple complex in the presence of BCA. The complex has peak absorbances at 562 and 354.5 nm with molar absorptivities of 7.7 x 10(3) liter mol(-1) cm(-1) and 4.6 X 10(4) liter mol(-1) cm(-1), respectively. Interference by other metal ions, pH, and detergents is minimal and the results correlate strongly with atomic absorption spectrophotometry. (C) 1995 Academic Press, Inc.

引用

页码：80 / 84

页数：5

共 10 条

[1] ABE A, 1989, CLIN CHEM, V35, P552
[2] Blair D., 1961, TALANTA, V7, P163
[3] GINDLER EM, 1970, CLIN CHEM, V6, P536
[4] Linder, 1991, BIOCH COPPER
[5] A SENSITIVE, DIRECT COLORIMETRIC ASSAY OF SERUM COPPER USING 5-BR-PSAA
MAKINO, T
[J]. CLINICA CHIMICA ACTA, 1989, 185 (01) : 7 - 16
[6] MUSTAFIN IS, 1965, ZH ANAL KHIM, V19, P190
[7] SPECTROPHOTOMETRIC DETERMINATION OF SERUM COPPER WITH BISCYCLOHEXANONEOXALYLDIHYDRAZONE
PETERSON, RE
BOLLIER, ME
[J]. ANALYTICAL CHEMISTRY, 1955, 27 (07) : 1195 - 1197
[8] ANALYSIS AND EVALUATION OF ZINC AND COPPER IN HUMAN-PLASMA AND SERUM
SMITH, JC
HOLBROOK, JT
DANFORD, DE
[J]. JOURNAL OF THE AMERICAN COLLEGE OF NUTRITION, 1985, 4 (06) : 627 - 638
[9] SMITH PK, 1985, ANAL BIOCHEM, V26, P76
[10] TIKHONOV VN, 1965, ZH ANAL KHIM, V20, P358

← 1 →