CAPILLARY ELECTROPHORETIC SEPARATION OF PROTEINS USING STABLE, HYDROPHILIC POLY(ACRYLOYLAMINOETHOXYETHANOL)-COATED COLUMNS

被引：70

作者：

CHIARI, M ^{[1
]}

NESI, M ^{[1
]}

SANDOVAL, JE ^{[1
]}

PESEK, JJ ^{[1
]}

机构：

[1] SAN JOSE STATE UNIV,DEPT CHEM,SAN JOSE,CA 95192

来源：

JOURNAL OF CHROMATOGRAPHY A | 1995年 / 717卷 / 1-2期

关键词：

D O I：

10.1016/0021-9673(95)00413-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

Capillary electrophoretic separations of high efficiency and resolution were obtained using poly(acryloylaminoethoxyethanol)-coated capillaries. The polymer was covalently attached to a silica surface previously modified with a alpha-methacryloxylpropyl functionality. The latter was realized by catalytic hydrosilylation of allyl methacrylate on an SiH-modified fused-silica capillary. The lifetime of the new type of coating used at pH 8.5 was more than twice that of conventional polyacrylamide.

引用

页码：1 / 13

页数：13

共 34 条

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