INTERLEUKIN-4 REGULATES INDUCTION OF SIALOADHESIN, THE MACROPHAGE SIALIC ACID-SPECIFIC RECEPTOR

被引:28
作者
MCWILLIAM, AS [1 ]
TREE, P [1 ]
GORDON, S [1 ]
机构
[1] UNIV OXFORD, SIR WILLIAM DUNN SCH PATHOL, OXFORD OX1 3RE, ENGLAND
关键词
D O I
10.1073/pnas.89.21.10522
中图分类号
O [数理科学和化学]; P [天文学、地球科学]; Q [生物科学]; N [自然科学总论];
学科分类号
07 ; 0710 ; 09 ;
摘要
Sialoadhesin is a nonphagocytic lectin-like receptor found on a restricted population of tissue macrophages in lymphoid and hemopoietic tissues. In bone marrow, it is localized to areas of contact between the resident stromal macrophages and developing granulocytes, which together form myeloblastic clusters. Sialoadhesin is highly specific for sialylated glycoconjugates and may play a role in adhesion and trophic hemopoietic cell interactions, although its function is unknown. Resident peritoneal macrophages do not express high levels of sialoadhesin in vitro unless an inducing element found in normal mouse serum is present. The restricted in vivo location of this marker and its induction by mouse serum prompted us to examine the possible influence of various cytokines on its expression, measured by a sheep erythrocyte rosetting assay. None of the cytokines tested was able to induce sialoadhesin; however, interleukin 4 (IL-4) prevented the induction in the presence of serum. Expression of other macrophage markers was not influenced in parallel, and Western blotting showed that sialoadhesin antigen in cell lysates was selectively reduced by IL-4. Inhibition by IL-4 was dose dependent, could be blocked by antibodies to both IL-4 and the IL-4 receptor, and was overcome by increased serum concentrations. IL-4 is therefore a potent cytokine regulator of the sialic acid-specific receptor implicated in macrophage-hemopoietic cell interactions.
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页码:10522 / 10526
页数:5
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