SACCHAROMYCES-CEREVISIAE PHOSPHOGLUCOSE ISOMERASE AND FRUCTOSE BISPHOSPHATE ALDOLASE CAN BE REPLACED FUNCTIONALLY BY THE CORRESPONDING ENZYMES OF ESCHERICHIA-COLI AND DROSOPHILA-MELANOGASTER

被引：35

作者：

BOLES, E ^{[1
]}

ZIMMERMANN, FK ^{[1
]}

机构：

[1] TH DARMSTADT,INST MICROBIOL,SCHNITTSPAHNSTR 10,W-6100 DARMSTADT,GERMANY

来源：

CURRENT GENETICS | 1993年 / 23卷 / 03期

关键词：

PHOSPHOGLUCOSE ISOMERASE; FRUCTOSE-1,6-BISPHOSPHATE ALDOLASE; HETEROLOGOUS GENE EXPRESSION; MULTIENZYME AGGREGATES;

D O I：

10.1007/BF00351494

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Two glycolytic enzymes, phosphoglucose isomerase and fructose-1,6-bisphosphate aldolase, of Saccharomyces cerevisiae could be replaced by their heterologous counterparts from Escherichia coli and Drosophila melanogaster. Both heterologous enzymes, which show respectively little and no sequence homology to the corresponding yeast enzymes, fully restored wild-type properties when their genes were expressed in yeast deletion mutants. This result does not support notions of an obligatory formation of glycolytic multi-enzyme aggregates in yeast; nor does it support possible regulatory functions of yeast phosphoglucose isomerase.

引用

页码：187 / 191

页数：5

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