GENETIC IDENTIFICATION OF STAPHYLOCOCCUS-AUREUS BY POLYMERASE CHAIN-REACTION USING SINGLE-BASE-PAIR MISMATCH IN 16S RIBOSOMAL-RNA GENE

被引：19

作者：

SARUTA, K

HOSHINA, S

MACHIDA, K

机构：

[1] JIKEI UNIV,SCH MED,DEPT LAB MED,MINATO KU,TOKYO 105,JAPAN

[2] JIKEI UNIV,SCH MED,DEPT INTERNAL MED 2,MINATO KU,TOKYO 105,JAPAN

来源：

MICROBIOLOGY AND IMMUNOLOGY | 1995年 / 39卷 / 11期

关键词：

STAPHYLOCOCCUS AUREUS; POLYMERASE CHAIN REACTION; 16S RIBOSOMAL-RNA;

D O I：

10.1111/j.1348-0421.1995.tb03280.x

中图分类号：

R392 [医学免疫学]; Q939.91 [免疫学];

学科分类号：

100102 ;

摘要：

Staphylococcus aureus is the most predominant and important pathogen in clinical microbiology. A DNA amplification assay using the polymerase chain reaction (PCR) was designed to identify S. aureus through a single-base-pair mismatch in the sequences of staphylococcal 16S ribosomal RNA (16S rRNA) genes. It was able to detect and identify S. aureus without requiring additional analytical techniques. Twenty-eight staphylococcal and non-staphylococcal strains were tested to verify the specificity of the assay, and only S. aureus strains gave a positive reaction. It may be possible to provide immediate and exact information for the identification of S. aureus.

引用

页码：839 / 844

页数：6

共 21 条

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