RELIABILITY OF POLYMERASE CHAIN-REACTION (PCR) ANALYSIS OF SINGLE CELLS FOR PREIMPLANTATION GENETIC DIAGNOSIS

被引：15

作者：

STROM, CM

RECHITSKY, S

WOLF, G

VERLINSKY, Y

机构：

[1] Reproductive Genetics Institute and Department of Obstetrics and Gynecology, Illinois Masonic Medical Center, Chicago, 60657, Illinois

来源：

JOURNAL OF ASSISTED REPRODUCTION AND GENETICS | 1994年 / 11卷 / 02期

关键词：

POLYMERASE CHAIN REACTION; GENETIC DIAGNOSIS; PREIMPLANTATION; SINGLE-LOCUS GENETIC DISEASE;

D O I：

10.1007/BF02215989

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Purpose: We investigated the reliability of polymerase chain reaction (PCR) genotype analyses performed on single cells for the purposes of preimplantation genetic analysis. Methods: We performed blind analysis of 130 single skin fibroblasts heterozygous for the DELTA-F508 mutation in the cystic fibrosis transmembrane regulator (CFTR) gene and 73 single skin fibroblasts from an individual heterozygous for the Xba polymoporphic site of the Factor VIII gene. Results: Amplification was successful for 116 cells and 52 cells respectively and in all but one case (a CFTR analysis) both alleles were amplified. The incidence of diagnostic error was 1 out of 203 analyses or 0.0043. We conclude that PCR is a reliable method for determining the genotype of single cells for the purposes of preimplantation genetic analysis.

引用

页码：55 / 62

页数：8

共 16 条

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