MECCSIM, TRAINING SOFTWARE FOR MICELLAR ELECTROKINETIC CAPILLARY CHROMATOGRAPHY

A training simulation program for micellar electrokinetic capillary chromatography was developed. The software provides a high speed of calculation, visual detail and model accuracy with a user-friendly interface. The instrument parameters that can be varied include capillary material, length, internal diameter and wall thickness, thermostating temperature, voltage and polarity. Hydrostatic injection is simulated using time and pressure as variables. The following properties of the buffer can be varied: pH, ionic strength, and sodium dodecylsulfate (SDS) concentration. The retention model is based on experimental values for delta H degrees and delta S degrees, obtained from the literature. In addition, the temperature and ionic strength dependence of the critical micelle concentration and the partial specific volume of the SDS micelle is taken into account. This allows the calculation of the temperature dependence of the partition coefficients and capacity factors and thus of the retention and migration behaviour. In addition, a number of dispersion factors are included in the model.