THE CA-MOV18 MOLECULE, A CELL-SURFACE MARKER OF HUMAN OVARIAN CARCINOMAS, IS ANCHORED TO THE CELL-MEMBRANE BY PHOSPHATIDYLINOSITOL

被引：36

作者：

ALBERTI, S ^{[1
]}

MIOTTI, S ^{[1
]}

FORNARO, M ^{[1
]}

MANTOVANI, L ^{[1
]}

WALTER, S ^{[1
]}

CANEVARI, S ^{[1
]}

MENARD, S ^{[1
]}

COLNAGHI, MI ^{[1
]}

机构：

[1] IST NAZL TUMORI,I-20133 MILAN,ITALY

来源：

BIOCHEMICAL AND BIOPHYSICAL RESEARCH COMMUNICATIONS | 1990年 / 171卷 / 03期

关键词：

D O I：

10.1016/0006-291X(90)90790-T

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

The struture of the 38 kD cell surface glycoprotein identified by the monoclonal antibody MOv18 and specifically expressed by human ovarian carcinomas has been investigated at a molecular level. The ovarian carcinoma cell line IGROV-1, which expresses high levels of Ca-MOv18, was treated with the phosphatidylinositol-specific phospholipase C from B. thuringiensis. The phospholipase C specifically released most of the Ca-MOv18 molecules as shown by flow cytometric analysis of the treated cells and by radioimmunometric assays of the corresponding supernatants. Consistent with the known structure of other phosphatidylinositol-linked molecules, Ca-MOv18 was biosynthetically labeled by [3H]ethanolamine and the labeled molecules were immunoprecipitated from the supernatant of the phospholipase C treated cells. Evidence that Ca-MOv18 is anchored to the cell membrane via phosphatidylinositol may prove to be relevant in current investigations regarding the biological and clinical significance of this tumor marker. © 1990.

引用

页码：1051 / 1055

页数：5

共 20 条

[1] DNA METHYLATION PREVENTS TRANSFECTION OF GENES FOR SPECIFIC SURFACE-ANTIGENS [J].