CHANGES IN SAMPLE ISOFERRITIN COMPOSITION AS A POSSIBLE CAUSE OF DILUTIONAL DISCREPANCIES IN FERRITIN 2-SITE IMMUNORADIOMETRIC ASSAY RESULTS

被引：1

作者：

RYALL, RG ^{[1
]}

RUMPFF, J ^{[1
]}

机构：

[1] FLINDERS UNIV S AUSTRALIA, MED CTR, DEPT HAEMATOL, BEDFORD PK, SA 5042, AUSTRALIA

来源：

ANNALS OF CLINICAL BIOCHEMISTRY | 1986年 / 23卷

关键词：

D O I：

10.1177/000456328602300206

中图分类号：

R446 [实验室诊断]; R-33 [实验医学、医学实验];

学科分类号：

1001 ;

摘要：

The effect of changes in sample isoferritin composition on the behavior of ferritin 2-site immunoradiometric assays was investigated using a computer model and experimental studies. Modelling studies predicted that under conditions where a sample has a different isoferritin composition from the assay standards, progressively higher values will be generated for the apparent ferritin content of the sample when it is analysed at increasing dilutions. In addition, the assay results will underestimate the actual ferritin content of such samples at all dilutions. Dilutional discrepancies in assay results and underestimation of sample ferritin concentrations were found in practical assays when synthetic samples with demonstrably different isoferritin profiles were analysed. Differences in isoferritin composition between assay standards and assayed samples may therefore be a cause of dilutional discrepancies in some results from ferritin 2-site immunoradiometric assays.

引用

页码：172 / 179

页数：8

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