ORGANIC-SOLVENT CHANGES THE CHYMOTRYPSIN SPECIFICITY WITH RESPECT TO NUCLEOPHILES

被引：27

作者：

GOLOLOBOV, MY

VOYUSHINA, TL

STEPANOV, VM

ADLERCREUTZ, P

机构：

[1] UNIV LUND,CTR CHEM,DEPT BIOTECHNOL,POB 124,S-22100 LUND,SWEDEN

[2] MOSCOW GENET & SELECT IND MICROORGAN INST,MOSCOW 113545,USSR

来源：

FEBS LETTERS | 1992年 / 307卷 / 03期

关键词：

ALPHA-CHYMOTRYPSIN; LOW WATER SYSTEM; NUCLEOPHILE SPECIFICITY; QSAR;

D O I：

10.1016/0014-5793(92)80702-I

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

In alpha-chymotrypsin-catalyzed acyl-transfer reactions in water the specificity of the enzyme (the nucleophile reactivity of amino acid amides) is correlated with the substrate hydrophobicity and increases as the hydrophobicity of the side chain of the amino acid amides is increased. In a low water system (4% H2O) bulky amino acid amides are less efficient nucleophiles. The specificity of alpha-chymotrypsin towards the amino acid amides in acyl transfer reactions in this case does not depend on the hydrophobicity of the amino acid side chains but correlates with their size. Therefore, different factors can be responsible for the specificity of enzymes in water and in a mainly organic medium.

引用

页码：309 / 312

页数：4

共 27 条

[1] ON THE IMPORTANCE OF THE SUPPORT MATERIAL FOR ENZYMATIC-SYNTHESIS IN ORGANIC MEDIA - SUPPORT EFFECTS AT CONTROLLED WATER ACTIVITY [J].