HIGH-LEVEL EXPRESSION OF A CHIMERIC ANTIGANGLIOSIDE GD2 ANTIBODY - GENOMIC KAPPA SEQUENCES IMPROVE EXPRESSION IN COS AND CHO CELLS

被引：34

作者：

FOUSER, LA

SWANBERG, SL

LIN, BY

BENEDICT, M

KELLEHER, K

CUMMING, DA

RIEDEL, GE

机构：

[1] Genetics Institute, Cambridge, MA, 02140

来源：

BIO-TECHNOLOGY | 1992年 / 10卷 / 10期

关键词：

D O I：

10.1038/nbt1092-1121

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

We report a flexible strategy for the high level expression of a recombinant human monoclonal antibody (mAb) in Chinese hamster ovary (CHO) cells, initially using COS monkey kidney cell transfections to evaluate rapidly modifications to immunoglobulin (Ig) DNA constructs. Using sequential transfections with two amplifiable markers, we generated CHO cell lines and clones that secrete 80-110 mug/10(6) cells/24 hours of a mouse-human chimeric IgG1kappa mAb. This cellular productivity is considerably greater than most murine hybridomas and transfected myelomas. Our data also demonstrate that genomic kappa sequences can improve mAb expression in COS and CHO cells. As a paradigm, we focused our expression studies on a human chimeric form of 3F8, a murine mAb that binds to ganglioside GD2 on neuroblastoma and melanoma tumor cells.

引用

页码：1121 / 1127

页数：7

共 61 条

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