A MULTIFUNCTIONAL PLASMID FOR PROTEIN EXPRESSION BY ECPCR - OVERPRODUCTION OF THE P50 SUBUNIT OF NF-KAPPA-B

被引：45

作者：

SODEOKA, M

LARSON, CJ

CHEN, L

LECLAIR, KP

VERDINE, GL

机构：

[1] HARVARD UNIV,DEPT CHEM,CAMBRIDGE,MA 02138

[2] MIT,DEPT BIOL,CAMBRIDGE,MA 02139

来源：

BIOORGANIC & MEDICINAL CHEMISTRY LETTERS | 1993年 / 3卷 / 06期

关键词：

D O I：

10.1016/S0960-894X(00)80293-0

中图分类号：

R914 [药物化学];

学科分类号：

100701 ;

摘要：

We report the construction of a new T7-based transcription vector, pLM1, designed for use in the ECPCR method of protein overproduction. The utility of pLM1 is illustrated by overproduction of the 50 kDa DNA-binding subunit of the human transcription factor NF-kappaB in E. coli. pLM1-p50 directs high-level expression of soluble, active NF-kappaB p50.

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页码：1089 / 1094

页数：6

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