THE PREPARATION OF BIOLOGICAL TISSUE FOR A TRACE-ELEMENT ANALYSIS ON THE PROTON MICROPROBE

被引：15

作者：

KIRBY, BJ ^{[1
]}

LEGGE, GJF ^{[1
]}

机构：

[1] UNIV MELBOURNE, MARC, PARKVILLE, VIC 3052, AUSTRALIA

来源：

NUCLEAR INSTRUMENTS & METHODS IN PHYSICS RESEARCH SECTION B-BEAM INTERACTIONS WITH MATERIALS AND ATOMS | 1993年 / 77卷 / 1-4期

基金：

澳大利亚研究理事会;

关键词：

D O I：

10.1016/0168-583X(93)95554-I

中图分类号：

TH7 [仪器、仪表];

学科分类号：

0804 ; 080401 ; 081102 ;

摘要：

The importance of careful specimen preparation techniques is to be appreciated if the results of an elemental microanalysis of a biological tissue section are to be an accurate representation of the tissue in vivo. The preservation of elemental distributions and tissue morphology, rapid excision and freeze fixation, followed by meticulous cryomicrotomy and freeze drying of the tissue section will be discussed. The production of artefacts in a fragile specimen in steps prior to and during analysis in a microprobe is to be minimised whenever possible.

引用

页码：268 / 274

页数：7

共 66 条

[1] PREPARATION OF THIN CRYO-SECTIONS FOR ELECTRON-PROBE ANALYSIS OF CALCIFYING CARTILAGE [J].