MOLECULAR-CLONING OF THE LARGE SUBUNIT OF GLUTATHIONE SYNTHETASE FROM XENOPUS-LAEVIS EMBRYOS

被引：16

作者：

HABENICHT, A

HILLE, S

KNOCHEL, W

机构：

[1] UNIV ULM,BIOCHEM ABT,OBERER ESELSBERG,W-7900 ULM,GERMANY

[2] FREE UNIV BERLIN,INST MOLEK BIOL & BIOCHEM,W-1000 BERLIN 33,GERMANY

来源：

BIOCHIMICA ET BIOPHYSICA ACTA | 1993年 / 1174卷 / 03期

关键词：

CDNA SEQUENCE; GLUTATHIONE SYNTHETASE; (XENOPUS-LAEVIS);

D O I：

10.1016/0167-4781(93)90202-O

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

We have isolated a cDNA clone from a Xenopus laevis tadpole cDNA library which probably codes for the large subunit of glutathione synthetase. The corresponding protein comprises 474 amino acids and shows a significant homology with the large subunit of glutathione synthetase of Schizosaccharomyces pombe. RNase protection experiments revealed that the gene is transcribed during oogenesis and that zygotic expression starts after midblastula transition. Transcripts are also detected in varios adult tissues suggesting an ubiquitous distribution of the corresponding protein.

引用

页码：295 / 298

页数：4

共 7 条

[1]

DAYHOFF MD, 1972, ATLAS PROTEIN SEQUEN, V5, P98

[2]

DOLPHINS D, 1988, GLUTATHIONE COENZYME

[3] COMPLETE NUCLEOTIDE-SEQUENCE OF THE ESCHERICHIA-COLI GLUTATHIONE SYNTHETASE GSH-II [J].