LIS2, GENE AND PSEUDOGENE, HOMOLOGOUS TO LIS1 (LISSENCEPHALY-1), LOCATED ON THE SHORT AND LONG ARMS OF CHROMOSOME-2

We report here the isolation of a novel cDNA, designated LIS2, that maps to chromosome 2p11.2 by in situ hybridization and demonstrates extremely high sequence similarity to the recently identified LIS1 gene involved in Miller-Dieker lissencephaly at 17p13.3. Specific probes for LIS2 revealed a pattern of expression resembling that of LIS1, although LIS2 is less abundant. Surprisingly, LIS2 detected an additional, higher molecular weight transcript in adult skeletal muscle. Isolated YAC clones and P1 clones mapped by in situ hybridization to two loci on chromosome 2, 2p11.2 and 2q13-q14. This hybridization was due to the existence of LIS2 pseudogene LIS2P on the long arm of chromosome 2. (C) 1995 Academic Press, Inc.