THE GRB2 BINDING DOMAIN OF MSOS1 IS NOT REQUIRED FOR DOWNSTREAM SIGNAL-TRANSDUCTION

被引：60

作者：

WANG, W

FISHER, EMC

JIA, Q

DUNN, JM

PORFIRI, E

DOWNWARD, J

EGAN, SE

机构：

[1] HOSP SICK CHILDREN,DIV IMMUNOL & CANC RES,TORONTO,ON M5G 1X8,CANADA

[2] ST MARYS HOSP,SCH MED,DEPT BIOCHEM & MOLEC GENET,LONDON W2 1PG,ENGLAND

[3] EYE RES INST CANADA,TORONTO,ON M5T 2S8,CANADA

[4] ONYX PHARMACEUT,RICHMOND,CA 94806

[5] IMPERIAL CANC RES FUND,SIGNAL TRANSDUCT LAB,LONDON WC2A 3PX,ENGLAND

来源：

NATURE GENETICS | 1995年 / 10卷 / 03期

关键词：

D O I：

10.1038/ng0795-294

中图分类号：

Q3 [遗传学];

学科分类号：

071007 ; 090102 ;

摘要：

Cellular Ras proteins are activated primarily by specific guanine-nucleotide releasing factors such as the Son of Sevenless (Sos) proteins. This activation event is thought to occur in response to plasma membrane localization of a complex containing Sos and a small adapter protein Grb2. We have isolated a dominant mutant allele of mSos1 which transforms Rat1 cells, yet is no longer able to bind Grb2. Biochemical experiments reveal that the subcellular distribution of this truncated Sos protein is not altered with respect to the wild type Sos protein. These data argue against a role for Grb2 in the direct recruitment of Sos proteins to the plasma membrane and suggest that Grb2 may function to overcome negative regulation of Sos by its C terminus.

引用

页码：294 / 300

页数：7

共 44 条

[1] MEMBRANE TARGETING OF THE NUCLEOTIDE EXCHANGE FACTOR SOS IS SUFFICIENT FOR ACTIVATING THE RAS SIGNALING PATHWAY [J].