KINETICS OF CHOLESTEROL OXIDASE SYNTHESIS BY NOCARDIA-RHODOCROUS

被引：38

作者：

BUCKLAND, BC ^{[1
]}

LILLY, MD ^{[1
]}

DUNNILL, P ^{[1
]}

机构：

[1] UCL, DEPT CHEM ENGN, BIOCHEM ENGN SECT, LONDON WC1E 7JE, ENGLAND

来源：

BIOTECHNOLOGY AND BIOENGINEERING | 1976年 / 18卷 / 05期

关键词：

D O I：

10.1002/bit.260180502

中图分类号：

Q81 [生物工程学（生物技术）]; Q93 [微生物学];

学科分类号：

071005 ; 0836 ; 090102 ; 100705 ;

摘要：

The production of cholesterol oxidase by 3 l batch cultures of N. rhodocrous growing on a glycerol/yeast extract medium was investigated. Cholesterol was a good inducer of the enzyme. The optimum time for cholesterol addition and the quantity to be added were determined, resulting in a 15-fold yield increase. Cholesterol oxidase synthesis was influenced by the dissolved O2 tension. Maximum cholesterol oxidase production was obtained at 30-40% air saturation. The effect of growth conditions on the extraction of cholesterol oxidase by Triton X-100 was investigated. The scale-up of the fermentation to 800 l in a pilot-plant fermenter is described.

引用

页码：601 / 621

页数：21

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