ARACHIDONIC-ACID AND DIACYLGLYCEROL ACT SYNERGISTICALLY TO ACTIVATE PROTEIN-KINASE-C INVITRO AND INVIVO

被引:102
作者
LESTER, DS
COLLIN, C
ETCHEBERRIGARAY, R
ALKON, DL
机构
[1] National Institutes of Health, Section of Neural Systems, Bethesda, MD 20892, Park 5 Building
关键词
D O I
10.1016/0006-291X(91)91745-X
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Using a well-defined model membrane bilayer system, incorporation of both lipid second messengers,1,2-diacylglycerol and arachidonic acid, at submaximal activating concentrations, resulted in a synergistic activation of protein kinase C in a Ca2+/phosphatidylserine-dependent manner as measured by monitoring phosphorylation of phosphoprotein substrates. The arachidonic acid appears to modulate membrane properties both at the hydrocarbon core and the membrane surface increasing the availability of the diacylglycerol which can bind to and subsequently activate the enzyme. Co-application of these two lipid activators to the Hermissenda photoreceptor reduced K+ channel conductance in a synergistic manner via a PKC-dependent pathway. Thus, these in vivo and in vitro studies suggest that the membrane bilayer properties of these PKC lipid activators interact to specifically regulate the cellular lipid microenvironment resulting in PKC activation. © 1991.
引用
收藏
页码:1522 / 1528
页数:7
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