THE USE OF ALKYLMETHYLMORPHOLINE-BASED BACKGROUND ELECTROLYTES IN CAPILLARY ELECTROPHORESIS

A homologous series of alkylmethylmorpholinium hydroxides has been synthesized, characterized and used as the source of cationic co-ions in mobility-matching background electrolytes designed to eliminate the efficiency-degrading effects of electromigration dispersion. The mobilities of the cc-ions vary over the 25*10(-5) to 45*10(-5) cm(2)NS range in 25 mM mobility-matching background electrolytes, prepared from phosphoric acid and the respective alkylmethylmorpholinium hydroxides. It was demonstrated experimentally that electromigration dispersion-related extra peak broadening could be minimized and difficult electrophoretic separations could be realized when these alkylmethylmorpholinium cations were used as co-ions.