IDENTIFICATION OF A HEAT-SHOCK PROMOTER IN THE TOPA GENE OF ESCHERICHIA-COLI

被引：36

作者：

LESLEY, SA

JOVANOVICH, SB

TSEDINH, YC

BURGESS, RR

机构：

[1] UNIV WISCONSIN,MCARDLE LAB CANC RES,MADISON,WI 53706

[2] NEW YORK MED COLL,DEPT BIOCHEM & MOLEC BIOL,VALHALLA,NY 10595

来源：

JOURNAL OF BACTERIOLOGY | 1990年 / 172卷 / 12期

关键词：

D O I：

10.1128/jb.172.12.6871-6874.1990

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

The transcriptional activity of the topA gene which codes for topoisomerase I was examined. An in vitro assay determined that the P1 promoter was dependent on the σ32 subunit of RNA polymerase. The transcriptional activity of the four topA promoters was examined by nuclease S1 mapping of the transcripts during a heat shock. This σ32-dependent promoter was shown to function as a heat shock promoter, although topoisomerase I is not a heat shock protein. A possible method of compensation of transcription activity by the other promoters to maintain the level of topoisomerase I during heat shock is proposed.

引用

页码：6871 / 6874

页数：4

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