AN ENZYME-LINKED-IMMUNOSORBENT-ASSAY FOR THE MEASUREMENT OF HUMAN INTERLEUKIN-6

被引：65

作者：

IDA, N

SAKURAI, S

HOSAKA, T

HOSOI, K

KUNITOMO, T

MATSUURA, Y

KOHASE, M

机构：

[1] TORAY INDUSTRIES LTD,MISHIMA PLANTS,SHIZUOKA 411,JAPAN

[2] NATL INST HLTH,DEPT VET SCI,TOKYO 141,JAPAN

[3] NATL INST HLTH,CENT VIRUS DIAGNOST LAB,MUSASHI MURAYAMA,TOKYO 19012,JAPAN

来源：

JOURNAL OF IMMUNOLOGICAL METHODS | 1990年 / 133卷 / 02期

关键词：

ELISA; Interleukin-6;

D O I：

10.1016/0022-1759(90)90369-7

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

An enzyme-linked immunosorbent assay has been developed to measure human interleukin-6. The assay, based on an avidin-biotin amplified two-step sandwich method, is quick (requiring 4.5 h), sensitive (detecting 9.5 pg/ml) and satisfactory in reproducibility and specificity. It shows good correspondence with the results of bioassays, and it is not affected by serum and poasma components. These results indicate that this ELISA is suitable for application to clinical samples, which is a major advantage over the widely used bioassays. © 1990.

引用

页码：279 / 284

页数：6

共 21 条

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