IMMUNODETECTION METHODS FOR GRASS-POLLEN ALLERGENS ON WESTERN BLOTS

被引：11

作者：

ONG, EK ^{[1
]}

SUPHIOGLU, C ^{[1
]}

SINGH, MB ^{[1
]}

KNOX, RB ^{[1
]}

机构：

[1] UNIV MELBOURNE,SCH BOT,PARKVILLE,VIC 3052,AUSTRALIA

来源：

INTERNATIONAL ARCHIVES OF ALLERGY AND APPLIED IMMUNOLOGY | 1990年 / 93卷 / 04期

关键词：

D O I：

10.1159/000235263

中图分类号：

R392 [医学免疫学];

学科分类号：

100102 ;

摘要：

A comparison is made of eight different methods to detect allergenic proteins in Western blots of rye-grass pollen extracts. Horseradish peroxidase-based enhanced chemiluminescence (ECL) provides a sensitive method for the detection of allergenic proteins. The method has been modified to use more dilute solutions of ECL substrate to reduce the background, can be applied to a standard nitrocellulose membrane, and used with Kodak X-ray film. The assays can be performed rapidly, replacing use of radiolabelled probes. Increased resolution is obtained. This makes the method suitable for detection of cDNA clones on plaque lifts, and for rapid and specific purification of proteins following immunodetection on nitrocellulose membranes.

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收藏

页码：338 / 343

页数：6

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