DETECTION OF THE ETHYL-RADICAL AND PENTYL-RADICAL ADDUCTS OF ALPHA-(4-PYRIDYL-1-OXIDE)-N-TERT-BUTYLNITRONE IN RAT-LIVER MICROSOMES TREATED WITH ADP, NADPH AND FERRIC-CHLORIDE

被引：19

作者：

IWAHASHI, H ^{[1
]}

PARKER, CE ^{[1
]}

TOMER, KB ^{[1
]}

MASON, RP ^{[1
]}

机构：

[1] NIEHS,MOLEC BIOPHYS LAB,RES TRIANGLE PK,NC 27709

来源：

FREE RADICAL RESEARCH COMMUNICATIONS | 1992年 / 16卷 / 05期

关键词：

ETHYL RADICAL; PENTYL RADICAL; SPIN TRAPPING; MICROSOMAL OXIDATION; HPLC-EPR;

D O I：

10.3109/10715769209049182

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HPLC-EPR analyses of the reaction mixtures of microsomal suspensions incubated with ADP, ferric chloride, NADPH and α4-pyridyl-1-oxide)-N-tert-butylnitrone (4-POBN) were performed. In the elution pattern of the reaction mixture, three peaks (peaks 1, 2 and 3) were detected. The radical adducts (1 and 3) were identified as being the pentyl- and ethyl-radical adducts of 4-POBN by comparing their retention times with those of the authentic radical adducts. © 1992 Informa UK Ltd All rights reserved: reproduction in whole or part not permitted.

引用

页码：295 / 301

页数：7

共 34 条

[1] SPIN-TRAPPING STUDIES ON THE FREE-RADICAL PRODUCTS FORMED BY METABOLIC-ACTIVATION OF CARBON-TETRACHLORIDE IN RAT-LIVER MICROSOMAL FRACTIONS-ISOLATED HEPATOCYTES AND INVIVO IN THE RAT [J].