HOMODIMERIC AND HETERODIMERIC ARYL SULFOTRANSFERASES CATALYZE THE SULFURIC-ACID ESTERIFICATION OF N-HYDROXY-2-ACETYLAMINOFLUORENE

被引:33
作者
KIEHLBAUCH, CC [1 ]
LAM, YF [1 ]
RINGER, DP [1 ]
机构
[1] OKLAHOMA MED RES FDN, NOBLE CTR BIOMED RES, OKLAHOMA CITY, OK 73104 USA
关键词
D O I
10.1074/jbc.270.32.18941
中图分类号
Q5 [生物化学]; Q7 [分子生物学];
学科分类号
071010 ; 081704 ;
摘要
Three aryl sulfotransferases (ASTs) isolated from rat liver catalyze the sulfuric acid esterification of the carcinogen N-hydroxy-2-acetylaminofluorene (N-OH-2AAF), These three ASTs were separated by high resolution anion exchange chromatography and were designated Q1, Q2, and Q3. Q1 and Q2 had high N-OH-2AAF sulfonation activity, whereas Q3 showed low activity, Reversed phase high performance liquid chromatography/mass spectrometry analysis showed Q1 Q3 to be comprised of 33,945- and 35,675-Da protein subunits, Q1 contained only the 35,675-Da protein subunit, Q2 contained equal quantities of 33,945- and 35,675-Da subunits, and Q3 contained only the 33,945-Da subunit. The subunit compositions of Q1-Q3 were confirmed by immunochemical analysis, Size exclusion high performance Liquid chromatography confirmed that the active quaternary structure of the three isoenzymes was dimeric, Analysis of liver cytosols for the relative contributions of Q1-Q3 to total cytosolic N-OH-2AAF sulfotransferase activity indicated that Q1, Q2, and Q3 accounted for 44, 46, and 10% of the activity, respectively, These results demonstrate the existence of both homodimeric and heterodimeric aryl sulfotransferases and show that two ASTs, a homodimer of 35,675-Da subunits and a heterodimer of a 33,945- and a 35,675-Da subunit, are primarily responsible for hepatic N-OH-2AAF sulfotransferase activity.
引用
收藏
页码:18941 / 18947
页数:7
相关论文
共 38 条
[1]   SEX-DIFFERENTIATED AND GROWTH-HORMONE-REGULATED MITOINHIBITION IN RAT-LIVER DURING TREATMENT WITH 2-ACETYLAMINOFLUORENE AND PARTIAL-HEPATECTOMY IN THE RESISTANT HEPATOCYTE MODEL [J].
BLANCK, A ;
ERIKSSON, LC ;
GUSTAFSSON, JA ;
HALLSTROM, IP .
CARCINOGENESIS, 1991, 12 (07) :1259-1264
[2]   TYROSINE-ESTER SULFOTRANSFERASE FROM RAT-LIVER - BACTERIAL EXPRESSION AND IDENTIFICATION [J].
CHEN, X ;
YANG, YS ;
ZHENG, YQ ;
MARTIN, BM ;
DUFFEL, MW ;
JAKOBY, WB .
PROTEIN EXPRESSION AND PURIFICATION, 1992, 3 (05) :421-426
[3]  
Cleland W W, 1979, Methods Enzymol, V63, P103
[4]   MYC MEETS ITS MAX [J].
COLE, MD .
CELL, 1991, 65 (05) :715-716
[5]  
DEBAUN JR, 1970, CANCER RES, V30, P577
[6]  
DUFFEL MW, 1991, MOL PHARMACOL, V40, P36
[7]  
FARBER E, 1984, CANCER RES, V44, P5463
[8]   PURIFICATION OF HEPATIC N-HYDROXYARYLAMINE SULFOTRANSFERASES AND THEIR REGULATION BY GROWTH-HORMONE AND THYROID-HORMONE IN RATS [J].
GONG, DW ;
OZAWA, S ;
YAMAZOE, Y ;
KATO, R .
JOURNAL OF BIOCHEMISTRY, 1991, 110 (02) :226-231
[9]  
HAYES JD, 1994, J BIOL CHEM, V269, P20707
[10]  
HEWICK RM, 1981, J BIOL CHEM, V256, P7990