DETERMINATION OF NIFEDIPINE IN HUMAN SERUM BY GAS-CHROMATOGRAPHY MASS-SPECTROMETRY - VALIDATION OF THE METHOD AND ITS USE IN BIOAVAILABILITY STUDIES

被引：26

作者：

MARTENS, J

BANDITT, P

MEYER, FP

机构：

[1] Department of Clinical Pharmacology, University Hospital, Otto-von-Guericke University Magdeburg, D-39120 Magdeburg

来源：

JOURNAL OF CHROMATOGRAPHY B-BIOMEDICAL APPLICATIONS | 1994年 / 660卷 / 02期

关键词：

D O I：

10.1016/0378-4347(94)00289-4

中图分类号：

Q5 [生物化学];

学科分类号：

071010 ; 081704 ;

摘要：

A procedure for the determination of nifedipine in human serum is described. The light-sensitive substance is isolated from serum by liquid-liquid extraction and analyzed using capillary gas chromatography with a mass-selective detector. The validation of the method shows that the extraction recovery is ca. 85%, the limit of detection is 2 ng/ml and the standard deviations of the intra-day precision test range from 5.8 to 7.4% with respect to the concentration. The procedure is highly selective and sensitive. It is especially suited for bioavailability studies because of its stability and high sampling rate.

引用

页码：297 / 302

页数：6

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