DETECTION OF 5-HYDROXYMETHYL-2'-DEOXYURIDINE IN DNA FROM CALF THYMUS, HELA-CELLS, AND RAT-LIVER NUCLEI

被引：6

作者：

CATTLEY, RC ^{[1
]}

DIETZE, SR ^{[1
]}

RICHARDSON, FC ^{[1
]}

POPP, JA ^{[1
]}

机构：

[1] CHEM IND INST TOXICOL,RES TRIANGLE PK,NC 27709

来源：

NUCLEOSIDES & NUCLEOTIDES | 1990年 / 9卷 / 02期

关键词：

D O I：

10.1080/07328319008045130

中图分类号：

Q5 [生物化学]; Q7 [分子生物学];

学科分类号：

071010 ; 081704 ;

摘要：

HMdU in DNA was quantitated by radioimmunoassay following separation of enzymatic digests. In DNA prepared from calf thymus, HeLa cells, and rat liver nuclei, there was approximately 1 HMdU for every 105-106 deoxythymidines. Irradiation of calf thymus DNA and HeLa cells increased the level of HMdU. © 1990, Taylor & Francis Group, LLC. All rights reserved.

引用

页码：179 / 187

页数：9

共 23 条

[1] DIETARY CARCINOGENS AND ANTICARCINOGENS - OXYGEN RADICALS AND DEGENERATIVE DISEASES [J].

AMES, BN .

SCIENCE, 1983, 221 (4617) :1256-1264

[2]

BEISER SM, 1968, METHOD ENZYMOL, V12, P889

[3]

BOORSTEIN RJ, 1989, CANCER RES, V49, P1509

[4]

BOREK C, 1985, ROLE CHEM RAD ETIOLO, P303

[5] THYMINE GLYCOL AND THYMIDINE GLYCOL IN HUMAN AND RAT URINE - A POSSIBLE ASSAY FOR OXIDATIVE DNA DAMAGE [J].

CATHCART, R ;

SCHWIERS, E ;

SAUL, RL ;

AMES, BN .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (18) :5633-5637

[6] SYNTHESIS OF 5-SUBSTITUTED PYRIMIDINES VIA FORMALDEHYDE ADDITION [J].

CLINE, RE ;

FINK, RM ;

FINK, K .

JOURNAL OF THE AMERICAN CHEMICAL SOCIETY, 1959, 81 (10) :2521-2527

[7]

CONWAY JG, 1987, CANCER RES, V47, P4795

[8] IONIZING-RADIATION-INDUCED DAMAGE IN THE DNA OF CULTURED HUMAN-CELLS - IDENTIFICATION OF 8,5-CYCLO-2-DEOXYGUANOSINE [J].

DIZDAROGLU, M ;

DIRKSEN, ML ;

JIANG, H ;

ROBBINS, JH .

BIOCHEMICAL JOURNAL, 1987, 241 (03) :929-932

[9] DNA DAMAGE RELATED TO INCREASED HYDROGEN-PEROXIDE GENERATION BY HYPOLIPIDEMIC DRUG-INDUCED LIVER PEROXISOMES [J].

FAHL, WE ;

LALWANI, ND ;

WATANABE, T ;

GOEL, SK ;

REDDY, JK .

PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES, 1984, 81 (24) :7827-7830

[10] QUANTITATIVE-DETERMINATION OF THE 5-(HYDROXYMETHYL)URACIL MOIETY IN THE DNA OF GAMMA-IRRADIATED CELLS [J].

FRENKEL, K ;

CUMMINGS, A ;

SOLOMON, J ;

CADET, J ;

STEINBERG, JJ ;

TEEBOR, GW .

BIOCHEMISTRY, 1985, 24 (17) :4527-4533

← 1 2 3 →