INVITRO INSULIN-LIKE GROWTH FACTOR-I INTERACTION WITH CARTILAGE CELLS DERIVED FROM POSTNATAL ANIMALS

被引：29

作者：

DEMARQUAY, D ^{[1
]}

DUMONTIER, MF ^{[1
]}

TSAGRIS, L ^{[1
]}

BOURGUIGNON, J ^{[1
]}

NATAF, V ^{[1
]}

CORVOL, MT ^{[1
]}

机构：

[1] HOP NECKER ENFANTS MALAD,INSERM,U30,UNITE RECH BIOL & PATHOL CROISSANCE & DEV,TOUR TECH 6E ETAGE,F-75743 PARIS 15,FRANCE

来源：

HORMONE RESEARCH | 1990年 / 33卷 / 2-4期

关键词：

Chondrocyte culture; Collagen; IGF-I; IGF-I local production; RNA;

D O I：

10.1159/000181493

中图分类号：

R5 [内科学];

学科分类号：

1002 ; 100201 ;

摘要：

This paper reports data on the in vitro effects of insulin-like growth factor I (IGF-I) and basic fibroblast growth factor (bFGF) on the phenotypic expression of epiphyseal chondrocytes grown in serum-frcc (SF) culture medium. bFGF mostly stimulates chondrocyte DNA and inhibits sulfated proteoglycan synthesis and type II collagen mRNA. On the contrary, IGF-I is poorly mitogenic but strongly stimulates protein synthesis and type II collagen mRNA. In addition, IGF-I prevents the expression of type I collagen gene. Lastly, chondrocytes cultured in SF medium are able to locally produce IGF-I peptides. In conclusion, IGF-I and bFGF have opposite effects on the phenotypic expression of chondrocytes in vitro: BFGF is mostly mitogenic and IGF-I appears to be a differentiating factor. © 1990 S. Karger AG, Basel.

引用

页码：111 / 115

页数：5

共 26 条

[1] A NOVEL ROLE FOR SOMATOMEDIN-C IN THE CYTODIFFERENTIATION OF THE OVARIAN GRANULOSA-CELL [J].