NOVEL TRANSFER-RNA GENE ORGANIZATION IN THE 16S-23S INTERGENIC SPACER OF THE STREPTOCOCCUS-PNEUMONIAE RIBOSOMAL-RNA GENE-CLUSTER

被引：57

作者：

BACOT, CM ^{[1
]}

REEVES, RH ^{[1
]}

机构：

[1] FLORIDA STATE UNIV,DEPT BIOL SCI B142,TALLAHASSEE,FL 32306

来源：

JOURNAL OF BACTERIOLOGY | 1991年 / 173卷 / 13期

关键词：

D O I：

10.1128/jb.173.13.4234-4236.1991

中图分类号：

Q93 [微生物学];

学科分类号：

071005 ; 100705 ;

摘要：

Isoleucine and alanine tRNAs are encoded tandemly within the 16S-23S intergenic spacer of some eubacterial rRNA gene clusters. Southern hybridization analysis and DNA sequence analysis demonstrated a novel gene organization for an rRNA gene cluster on the Streptococcus pneumoniae chromosome. A sequence specifying an alanine tRNA was found within the intergenic spacer, but no sequence specifying an isoleucine tRNA was found there. Southern hybridization analysis indicated that the location of the isoleucine tRNA gene was near the 5S rRNA gene in two of four rRNA gene clusters.

引用

页码：4234 / 4236

页数：3

共 25 条

[1]

ANDERSON P, 1987, GENETICS, V15, P581

[2] ORGANIZATION OF THE RIBOSOMAL-RNA GENES IN STREPTOMYCES-COELICOLOR A3(2) [J].

BAYLIS, HA ;

BIBB, MJ .

MOLECULAR & GENERAL GENETICS, 1988, 211 (02) :191-196

[3] THE DISTAL END OF THE RIBOSOMAL-RNA OPERON RRND OF ESCHERICHIA-COLI CONTAINS A TRANSFER RNA1(THR) GENE, 2 5S RIBOSOMAL-RNA GENES AND A TRANSCRIPTION TERMINATOR [J].

DUESTER, GL ;

HOLMES, WM .

NUCLEIC ACIDS RESEARCH, 1980, 8 (17) :3793-3807

[4]

Ehrlich S. D., 1989, MOBILE DNA-UK, P799

[5] ORGANIZATION AND NUCLEOTIDE-SEQUENCE ANALYSIS OF AN RIBOSOMAL-RNA AND TRANSFER-RNA GENE-CLUSTER FROM CAULOBACTER-CRESCENTUS [J].

FEINGOLD, J ;

BELLOFATTO, V ;

SHAPIRO, L ;

AMEMIYA, K .

JOURNAL OF BACTERIOLOGY, 1985, 163 (01) :155-166

[6] AN IMPROVED DIPHENYLAMINE METHOD FOR ESTIMATION OF DEOXYRIBONUCLEIC ACID [J].

GILES, KW ;

MYERS, A .

NATURE, 1965, 206 (4979) :93-&

[7] UNIDIRECTIONAL DIGESTION WITH EXONUCLEASE-III CREATES TARGETED BREAKPOINTS FOR DNA SEQUENCING [J].

HENIKOFF, S .

GENE, 1984, 28 (03) :351-359

[8]

HUI I, 1985, J BIOL CHEM, V260, P899

[9] EXPRESSION OF SPACER TRANSFER-RNA GENES IN RIBOSOMAL-RNA TRANSCRIPTION UNITS CARRIED BY HYBRID COL E1 PLASMIDS IN ESCHERICHIA-COLI [J].

IKEMURA, T ;

NOMURA, M .

CELL, 1977, 11 (04) :779-793

[10] DNA-SEQUENCE OF THE 16S RIBOSOMAL-RNA 23S RIBOSOMAL-RNA INTERCISTRONIC SPACER OF 2 RDNA OPERONS OF THE ARCHAEBACTERIUM METHANOCOCCUS-VANNIELII [J].

JARSCH, M ;

BOCK, A .

NUCLEIC ACIDS RESEARCH, 1983, 11 (21) :7537-7544

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